›› 2009, Vol. 40 ›› Issue (6): 873-876.doi: 10.3969/j.issn.0529-1356.2009.06.005

• 论著 • 上一篇    下一篇

创伤后应激障碍大鼠蓝斑核受体-糖皮质激素受体表达的变化

李慢 ;韩芳; 石玉秀*   

  1. 中国医科大学基础医学院组织胚胎学教研室,沈阳 110001
  • 收稿日期:2009-01-20 修回日期:2009-03-20 出版日期:2009-12-06
  • 通讯作者: 石玉秀

Changes of nuclear receptors-glucocorticoid receptors in locus ceruleus neurons of posttraumatic stress disorder-like rat

  1. Department of Histology and Embryology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China
  • Received:2009-01-20 Revised:2009-03-20 Online:2009-12-06
  • Contact: SHI Yu-xiu

关键词: 创伤后应激障碍, 蓝斑, 核受体, 糖皮质激素受体, 免疫印迹法, 大鼠

Abstract: Objective To investigate expression changes of nuclear receptors-glucocorticoid receptors (GR) in the locus ceruleus neurons of posttraumatic stress disorder(PTSD)like rats. Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress (SPS). There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed. Results GR was distributed in the nucleus of coeruleus neurons, GR expression was showed after 24 hours, 4 days, 7 days treatment and gradually increased, restorative downregulation was seen after 14 days and 28 days, but still high(EM>P/EM><0.05). Conclusion After SPS, the GR locus ceruleus temporarily increased and then lowered, suggesting that PTSD rat locus coeruleus neurons in nuclear receptor-GR expression changes may directly involve in the continuing spirit of PTSD symptoms occurred in the development process

Key words: Posttraumatic stress disorders, Locus ceruleus, Nuclear receptors, Glucocorticoid receptors, Western blotting, Rat

中图分类号: